Southampton Under-21s' game away to their Reading counterparts will be broadcast live on BT Sport.
Due to the game being selected for TV, the kick-off time has moved to 7.45pm BST on Monday 21st September.
The Barclays Under-21 Premier League fixture will still take place at Wycombe Wanderers's Adams Park and will be the fifth league game for Martin Hunter’s side, who get their campaign under way against Manchester City at Staplewood on 10th August.
Hunter's side are currently midway through a pre-season training camp at St George’s Park, with the Under-18s and Under-21s both involved.
Following Monday’s defeat to the Nike Academy, Saints’ youngsters still have a chance to end their stay at England’s Official Training Complex with a win, as they face Derby County on Friday.
The Under-21s were scheduled to face Fulham on Monday 3rd August, but they will instead take on Brentford at Staplewood on Tuesday 4th August (kick-off 7pm BST).